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                                                                                                                                                                                                                                                                                	                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      %                           (                                                                                            (                                                           	                                                                 
                                              	   	                     
                  &   &                  	      	      	         	   
                     #      
                                             /         -   *         #         €   G         )   #      )   l                      /                                                                                                                                                                    	                                                                                                                                                                                                                                                                                                                            %         (                                  (                     	                       
                	 	       
      & &      	  	  	   	 
       #  
               /   - *   #   € G   ) #  ) l   z  {�  {§  {ó  €¡  …™  Œ+¬ i>V h %_¥NhÅ,LMm|}‘’•gkÌKLËR<WúX¼Öop‹3„™¹ºóss¾V†´Îd�×o‘á÷eâ‡P>a^ 
Å
{@ÇL˜ s
=º«5(	iUt
flou~ö™ádkËó u ±5ik•°Î(	F		ö¦™áci	ER~‡dT§Ó7‹Iúµ
š¾{V¦†´¾ã	{Vâà†´¾{V†´ã	ì»ZÁÎd–a ‚ÇùDpÃhusÎ<]y¤ˆskX.œ.h œs8–žµ\!Ê|ä0ç	¸&Ð)j�<IPÌÌ\!Ê¾£
›DTnŸ3	ð^ƒ¡�SðÕÔ×Á^­ƒ®wÛ 
o¤G·	¥3´Éû€#p÷	8
ò
ÇËþU8+mFˆÛDÔø
€MîF Š«ó€	^ã	­€^ªìQ]Zt}n‡Ç+fž{é€^TØYRÂ}¥´#é	^õã	­º(}žó [hÔžægŽðŸ	*O´aÔ	 lôü¬Ú:¡©øÔz“à(d ‹ �íé7öé	&Sðf…VÚÕ"Ô‚Ê×ñ‡”Á^­^|}ƒ[KLº¶° Ë/2G	5�¢wâ>æ •	 OYì,P�‚<=IJî3<PQÆ~ÆAdmžÐ\ÿNn½ÇZ™¨ §æùa¯×ÔJÁ	œÏ
=‹Ã8uœ¥3‡Ï‡v9]€Ä$C—2ÇG	¬3ºŠB¤•ÐA €	^õã	T­ºØYRÂ(}ayF Š«Y,<AžÇ™ ØU	x—’U z ª1ž¬×O	c	g	Á	w{œÏ£§Ôç"H‹žÓæ-N~ÂÉåé
=Ü@]ÊA A
LF Š«žæÇÒ(44Eé	à§ü(^JL‚hØ<ìZ;Nã
Z*Ž  \pƒ&0;¶ªã	­èVU8+[KmLFˆºÞ¯Ò1‰ÑÙ@ŸRñúHË èA
×
LpTn˜€h^;rƒ
A
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È
×
bLpíTËö�Dn/µyÌ¤	ö
M9ŽŽ-å^i¤R»§Ú:éMˆ
¼ƒ
„ƒKlü	
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<UUID>4f069019-56db-4ba7-88a8-662d44acfab9</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="5:54:4">2025.4.16</Created>
<LastModified UTC="5:56:30">2025.4.16</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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  e<Notes>
<UUID>4f069019-56db-4ba7-88a8-662d44acfab9</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="5:54:4">2025.4.16</Created>
<LastModified UTC="5:56:20">2025.4.16</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  l      c®ZTR
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This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7101-7117" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7125-7141" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="miR-30a loop" directionality="1" type="ncRNA" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4503-4517" color="#8fbc8f" type="standard"/><Q name="ncRNA_class"><V predef="miRNA"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;loop from the&amp;nbsp;the 71-nt precursor of the human miR-30a microRNA (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/12086629&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/12086629&quot;&gt;Zeng et al., 2002&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ¾<Notes>
<UUID>7d8b0898-dfaf-4da1-bbaa-0e7b50c5b0c7</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pT2-shp53-GFP4</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="5:54:4">2025.4.16</Created>
<LastModified UTC="7:17:58">2026.1.24</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         
?????                                                                                                       ÿ d      DO ÿþ              E              ÿÿÿÿYô? P                                                                                                                                                             û<StrandColors><TopStrand><ColorRange range="2768..2837" colors="black"/><ColorRange range="2838..2840" colors="blue"/><ColorRange range="2841..4319" colors="purple"/><ColorRange range="4320..5042" colors="blue"/><ColorRange range="5043..5763" colors="purple"/><ColorRange range="5764..5788" colors="blue"/><ColorRange range="5789..6653" colors="purple"/><ColorRange range="6654..7079" colors="orange"/><ColorRange range="7080..7083" colors="red"/><ColorRange range="7084..7155" colors="black"/></TopStrand><BottomStrand><ColorRange range="2768..2837" colors="black"/><ColorRange range="2838..2840" colors="blue"/><ColorRange range="2841..4319" colors="purple"/><ColorRange range="4320..5042" colors="blue"/><ColorRange range="5043..5763" colors="purple"/><ColorRange range="5764..5788" colors="blue"/><ColorRange range="5789..6653" colors="purple"/><ColorRange range="6654..7079" colors="orange"/><ColorRange range="7080..7083" colors="red"/><ColorRange range="7084..7155" colors="black"/></BottomStrand></StrandColors>   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
